[Due to the increasing size of the archives, each topic page now contains only the prior 365 days of content. Access to older stories is now solely through the Monthly Archive pages or the site search function.]
Berkeley Lab researchers advance hybrid bioinorganic approach to solar-to~chemicals conversion; 50% electrical-to-chemical, 10% solar-to-chemical efficiencies
August 25, 2015
A team of researchers at the US Department of Energy (DOE)’s Lawrence Berkeley National Laboratory (Berkeley Lab) have hit a new milestone in their development of a hybrid bioinorganic system for solar-to-chemical energy conversion. (Earlier post.) The system first generates renewable hydrogen from water splitting using sustainable electrical and/or solar input and biocompatible inorganic catalysts. The hydrogen is then used by living cells as a source of reducing equivalents for conversion of CO2 to the value-added chemical product methane.
The system can achieve an electrical-to-chemical efficiency of better than 50% and a solar-to-chemical energy conversion efficiency of 10% if the system is coupled with state-of-art solar panel and electrolyzer, said Peidong Yang, a chemist with Berkeley Lab’s Materials Sciences Division and one of the leaders of this study. A paper on their work is published in Proceedings of the National Academy of Sciences (PNAS).
Synbio company Intrexon and Dominion partner to commercialize bioconversion of natural gas to isobutanol in Marcellus and Utica Basins
August 20, 2015
Intrexon Energy Partners (IEP), a joint venture of synthetic biology company Intrexon Corporation and external investors (earlier post), and Dominion Energy, a subsidiary of Dominion Resources, have entered into an agreement to explore the potential for commercial-scale biological conversion of natural gas to isobutanol in the Marcellus and Utica Shale Basins.
Intrexon’s proprietary methanotroph bioconversion platform uses optimized microbial cell lines to convert natural gas into higher carbon compounds such as isobutanol and farnesene under ambient temperatures and pressures. This novel approach avoids costly, resource-intensive thermochemical gas-to-liquids (GTL) conversion methods, and offers a biofuel that does not utilize sugar or other plant-based feedstock.
PNNL study of metabolic processes paves way to optimize lipids production in yeast Y. lipolytica
Lipid-derived biofuels have been proposed as a promising substitute for fossil fuels. The oleaginous ascomycete (sac fungus) yeast Yarrowia lipolytica accumulates large amounts of lipids and has potential as a biofuel producing organism; however, little is known about the key biological processes involved. To address this gap in knowledge, a recent study by a team from the Pacific Northwest National Laboratory (PNNL) identified and characterized major pathways involved in lipid accumulation from glucose in Y. lipolytica.
This study builds a platform for efforts to engineer the yeast to optimize lipid accumulation and maximize the yield of carbon-based products. Because lipids from Y. lipolytica have chemical properties similar to those of diesel fuel, they can be readily used as biodiesel using current vehicles and existing infrastructure at gas stations. Thus, harnessing lipids from Y. lipolytica could represent a practical approach for transitioning more quickly to a biofuel-based energy system.
Researchers modify camelina to produce highest levels yet in transgenic plant oil of novel lipid acetyl-TAG; biofuel and industrial use
August 18, 2015
Researchers at Kansas State University led by Professor Timothy Durrett and their colleagues at Michigan State University and the University of Nebraska, Lincoln have engineered Camelina sativa—a non-food oilseed crop—to produce high levels (up to 85 mol%) of acetyl-triacylglycerols (acetyl-TAGs, or ac-TAGs)—a novel plant oil lipid with possible biofuel or industrial uses.
As reported in a paper in Plant Biotechnology Journal, this successful metabolic engineering and subsequent field production of the modified camelina crop marked the highest accumulation of the unusual oil achieved so far in transgenic plants. (Earlier work by Durrett and colleagues at the DOE Great Lakes Bioenergy Research Center had resulted in approximately a 60 mol% accumulation of ac-TAGs.)
Researchers propose 2nd law of thermodynamics-based process to select and develop microorganisms for optimal biofuel production
August 17, 2015
Researchers at the University of Maryland are proposing a new process to isolate and to direct the evolution of microorganisms that convert cellulosic biomass or gaseous CO2 and H2 to biofuels such as ethanol, 1-butanol, butane, or hexane (among others).
The approach is based on the theory that fermentation systems drive toward thermodynamic equilibrium. Physical chemists, observe Richard Kohn and Seon-Woo Kim, both of the Department of Animal and Avian Sciences, in their paper published in the Journal of Theoretical Biology, have understood that all chemical reactions are controlled by either thermodynamic or kinetic mechanisms. With thermodynamic control, the feasibility of reactions and the availability of pathway branches depend on the second law of thermodynamics. This law governs whether or not a reaction can proceed spontaneously in the forward direction based on the concentrations of reactants and products.
U Georgia team discovers tungsten in novel bacterial enzyme; potential for cellulosic biofuels
August 16, 2015
A team at the University of Georgia, Athens led by Distinguished Research Professor Michael Adams has discovered tungsten in what appears to be a novel enzyme in the biomass-degrading thermophilic bacterium Caldicellulosiruptor bescii. Tungsten is exceptionally rare in biological systems.
The researchers hypothesized that this new tungstoenzyme plays a key role in C. bescii’s primary metabolism, and its ability to convert plant biomass to simple fermentable sugars. This discovery could ultimately lead to commercially viable conversion of cellulosic biomass to fuels and chemical feedstocks. The research is published in Applied and Environmental Microbiology, a journal of the American Society for Microbiology.
DOE BESC engineered microbe improves biobutanol yield from cellulose by a factor of 10
August 14, 2015
Researchers at the US Department of Energy’s (DOE’s) BioEnergy Science Center (BESC) have engineered a microbe that improves isobutanol yields from cellulose by a factor of 10. The work, published in the journal Metabolic Engineering, builds on results from 2011 in which researchers reported on the first genetically engineered microbe to produce isobutanol directly from cellulose. (Earlier post.)
Isobutanol is attractive because its energy density and octane values are closer to those of gasoline; it is useful not only as a direct replacement for gasoline but also as a chemical feedstock for a variety of products. For example, isobutanol can be chemically upgraded into a hydrocarbon equivalent for jet fuel.
New one-pot process to produce gasoline-grade biofuel from the bacterial biopolymer PHB
August 09, 2015
A team from the Hawaii Natural Energy Institute, University of Hawaii at Manoa is developing a new one-pot process to produce gasoline-grade (C6–C18) hydrocarbon oil from polyhydroxybutyrate (PHB)—an energy storage material formed from renewable feedstock in many bacterial species. In contrast to conventional biofuels derived from plant biomass, the resultant PHB oil has a high content of alkenes or aromatics, depending on the catalyst.
PHB has already been identified as having great potential as an intermediate in the production of hydrocarbon fuels. One approach, described by a team from the National Renewable Energy Laboratory (Wang et al.), is thermally to depolymerize and decarboxylate PHB at 400 ˚C to propene, for subsequent upgrading to hydrocarbon fuels via commercial oligomerization technologies.
DOE JGI team identify regulators of lipid production in algae; potential boost for algal fuels development
August 04, 2015
Algae naturally produce oils that can be converted into transportation fuels, making this a potentially attractive pathway for large-scale biofuel production. However, high-yield lipid production in algae is a stress response—induced, for example, through conditions such as nutrient deprivation. One of the challenges of optimizing this oil production pathway has been stressing the algae just enough to produce lipids in high yields, but not stressing them enough to kill them.
Now, a team led by scientists from the US Department of Energy Joint Genome Institute (DOE JGI) has analyzed the genes that are being activated during algal lipid production, and in particular the molecular machinery that orchestrates these gene activities inside the cell when it produces lipids. The work, published in a paper in the journal Nature Plants, may help algal bioenergy researchers develop more targeted approaches for producing lipids for fuels.
Researchers engineer first low-methane-emission, high-starch rice; benefits for GHG control, food and bioenergy
July 30, 2015
Rice—the staple food for more than half of the world’s population—is one of the largest manmade sources of atmospheric methane, a potent greenhouse gas. Now, however, with the addition of a single gene from barley (SUSIBA2), a team of researchers in China, Sweden and the US has engineered a strain of rice—now named SUSIBA2—that can be cultivated to emit virtually no methane from its paddies during growth.
The new strain also delivers much more of the plant’s desired properties, such as starch for a richer food source and biomass for energy production. SUSIBA2 rice is the first high-starch, low-methane rice that could offer a significant and sustainable solution. A paper on the work is published in the journal Nature.
ArcelorMittal, LanzaTech and Primetals to build €87M commercial-scale waste-gas-to-ethanol plant
July 13, 2015
ArcelorMittal, the world’s leading steel and mining company; LanzaTech; and Primetals Technologies, a leading technology and service provider to the iron and steel industry have entered into a letter of intent to construct Europe’s first commercial-scale production facility to create bioethanol from waste gases produced during the steelmaking process. (Earlier post.)
The €87-million (US$96 million) plant will produce 47,000 tons (about 15.7 million gallons US, 60 million liters) per year of ethanol. The resulting bioethanol can cut greenhouse gas emissions by more than 80% compared with conventional fossil fuels. It will predominantly be used in gasoline blending, but it can also be further processed into other products such as drop in jet fuel.
Fraunhofer developing process to ferment steel exhaust gases to fuels and chemicals
July 02, 2015
Fraunhofer researchers in Germany have developed a process for the conversion of CO-rich exhaust gases from steel plants into fuels and specialty chemicals. With the aid of genetically modified strains of Clostridium, the research team ferments the gas into alcohols and acetone, converts both substances catalytically into a kind of intermediary diesel product, and from produce kerosene and special chemicals.
Participants include the Fraunhofer Institute for Molecular Biology and Applied Ecology IME in Aachen, as well as the Institute for Environment, Safety, and Energy Technology UMSICHT in Oberhausen and the Institute for Chemical Technology ICT in Pfinztal. The technology came about during one of Fraunhofer’s internal preliminary research projects and through individual projects with industrial partners. The patented process currently operates on the laboratory scale.
LanzaTech gas fermentation technology at core of €14.6M EU Steelanol project; 25K t/year demo plant
June 26, 2015
LanzaTech’s gas fermentation technology (earlier post) is at the core of the new Horizon2020 Steelanol project (2015-2018), which seeks to produce bioethanol via an innovative gas fermentation process using exhaust gases emitted by the steel industry. The €14.6-million (US$16.3-million) project is coordinated by steelmaker Arcelormittal Belgium NV.
Steelanol’s main objective is to demonstrate the cost-effective production of sustainable bioethanol, with the purpose of assessing the valorization of this ethanol biofuel as a fuel derivative for the transport sector. The project will build a demonstration plant of approximately 25,000 tons/ethanol per year—the first of its kind in Europe, and the largest facility globally built to date utilizing this technology.
Volkswagen AG coordinating new €6M EU research project on drop-in biocatalytic solar fuels
Volkswagen AG is coordinating a new €6-million (US$6.7-million) research project, selected for funding under the Horizon 2020 Programme, to advance the biocatalytic production of drop-in liquid hydrocarbon transportation fuels, requiring only sunlight, CO2 and water.
The basic approach of the new 4-year Photofuel project is to develop and to advance microbes (the biocatalysts) that will directly excrete hydrocarbon and long-chain alcohol fuel compounds to the growth medium, from which the fuels are separated, without the need to harvest biomass. This basic concept is in line with the fundamental approach (CO2 + water + renewable energy → drop-in fuels) being taken by Audi (a member of the Volkswagen Group) in its e-fuels initiatives. (Earlier post.)
Joule issued patent on production of medium chain-length alkanes from sunlight and CO2; diesel, jet fuel and gasoline
June 16, 2015
Joule, the developer of engineered photosynthetic bacteria as catalysts for the direct production of targeted fuel molecules in a continuous, single-step conversion process, announced the issuance of an additional patent, extending its ability to target the highest-value molecules of the petroleum distillation process and generate them on demand from sunlight and CO2.
US Patent Nº 9,034,629, issued on 19 May, covers both the cyanobacterium and the process for directly converting CO2 into medium chain-length alkanes (C7-11), which are in the diesel, jet fuel and gasoline ranges.
New yeast engineered by BESC, Mascoma could accelerate production of cellulosic ethanol
June 04, 2015
Consolidated bioprocessing technology company Mascoma LLC and the US Department of Energy’s BioEnergy Science Center (BESC) have developed a new strain of yeast that could help significantly accelerate the development of biofuels from nonfood plant matter. The new C5 FUEL yeast delivers fermentation and ethanol yields that set a new standard for conversion of biomass sugars from pretreated corn stover, converting up to 97% of the plant sugars into fuel, the researchers said.
While conventional yeast leaves more than one-third of the biomass sugars unused in the form of xylose, Mascoma’s C5 FUEL efficiently converts this xylose into ethanol, and it accomplishes this feat in less than 48 hours. The results were presented at the 31st International Fuel Ethanol Workshop this week in Minneapolis.
WSU team engineers fungus to produce jet-range hydrocarbons from biomass
May 06, 2015
|Aspergillus carbonarius. Source: JGI MycoCosm. Click to enlarge.|
Researchers at Washington State University have engineered the filamentous fungus Aspergillus carbonarius ITEM 5010 to produce jet-range hydrocarbons directly from biomass. The researchers hope the work, reported in the journal Fungal Biology, leads to economically viable production of aviation biofuels in the next five years.
The team led by Birgitte Ahring, director and Battelle distinguished professor of the Bioproducts, Sciences and Engineering Laboratory at WSU Tri-cities, found that the production of hydrocarbons was dependent on the type of media used. Therefore, they tested ten different carbon sources (oatmeal, wheat bran, glucose, carboxymethyl cellulose, avicel, xylan, corn stover, switch grass, pretreated corn stover, and pretreated switch grass) to identify the maximum number and quantity of hydrocarbons produced.
China Steel Corporation making $46M investment in LanzaTech commercial waste-gas-to-ethanol project
April 22, 2015
Taiwan’s largest integrated steel maker, China Steel Corporation (CSC), has announced formal Board approval of a 1400-million TWD (US$46 million) capital investment in a LanzaTech commercial ethanol facility. This follows the successful demonstration of the carbon recycling platform at the White Biotech (WBT) Demonstration Plant in Kaohsiung using steel mill off gases for ethanol production.
LanzaTech’s gas fermentation process uses proprietary microbes to capture and reuse carbon rich waste gases, reducing emissions and pollutants from industrial processes such as steel manufacturing, while making fuels and chemicals that displace those made from fossil resources. (Earlier post.)
Researchers engineer new pathway in E. coli to produce renewable propane
April 15, 2015
Researchers at The University of Manchester, Imperial College London and University of Turku have made an advance toward the renewable biosynthesis of propane with the creation of a new synthetic pathway in E. coli, based on a fermentative butanol pathway. An open access paper on the work is published in the journal Biotechnology for Biofuels.
In 2014, members of the team from Imperial College and the University of Turku had devised a synthetic metabolic pathway for producing renewable propane from engineered E. coli bacteria, using pathways based on fatty acid synthesis. (Earlier post.) Although the initial yields were far too low for commercialization, the team was able to identify and to add essential biochemical components in order to boost the biosynthesis reaction, enabling the E. coli strain to increase propane yield. Yields, however, were still too low.
UC Berkeley hybrid semiconductor nanowire-bacteria system for direct solar-powered production of chemicals from CO2 and water
April 10, 2015
Researchers at UC Berkeley have developed an artificial photosynthetic scheme for the direct solar-powered production of value-added chemicals from CO2 and water using a two-step process involving a biocompatible light-capturing nanowire array with a direct interface with microbial systems.
As a proof of principle, they demonstrated that, using only solar energy input, such a hybrid semiconductor nanowire–bacteria system can reduce CO2 at neutral pH to a wide array of chemical targets, such as fuels, polymers, and complex pharmaceutical precursors A paper on their work is published in the ACS journal Nano Letters.
Virginia Tech team engineers optimized synthetic enzymatic pathway for high-yield production of H2 directly from biomass
April 07, 2015
A team of Virginia Tech researchers and colleagues has demonstrated the complete conversion of glucose and xylose from pretreated plant biomass to H2 and CO2 based on an in vitro synthetic enzymatic pathway crafted from more than 10 purified enzymes. Glucose and xylose were simultaneously converted to H2 with a yield of two H2 per carbon, the maximum possible yield.
The researchers used a nonlinear kinetic model fitted with experimental data to identify the enzymes that had the greatest impact on reaction rate and yield. After optimizing enzyme loadings using this model, volumetric H2 productivity was increased 3-fold to 32 mmol H2⋅L−1⋅h−1. The productivity was further enhanced to 54 mmol H2⋅L−1⋅h−1 by increasing reaction temperature, substrate, and enzyme concentrations—an increase of 67-fold compared with the initial studies using this method.
UT Austin researchers significantly boost yield and speed of lipids production from engineered yeast; more efficient biofuel production
March 24, 2015
Researchers in the Cockrell School of Engineering at The University of Texas at Austin have used a combination of metabolic engineering and directed evolution to develop a new strain of the yeast Yarrowia lipolytica featuring significantly enhanced lipids production that could lead to a more efficient biofuel production process. Their findings were published online in the journal Metabolic Engineering.
Beyond biofuels, the new yeast strain could be used in biochemical production to produce oleochemicals, chemicals traditionally derived from plant and animal fats and petroleum, which are used to make a variety of household products.
A*STAR team combines fungal culture and acid hydrolyses for cost-effective production of fermentable sugars from palm oil waste
March 16, 2015
After the harvest of the fruit from oil palm trees, large amounts of leftover biomass known as empty fruit bunch remain. The industry wants to use these leftover fruit bunches to produce bioethanol and biodegradable plastic, but has stumbled in their efforts to convert the leftovers in a cost-efficient way. The new fungal culture could make it possible to produce fermentable sugars from this huge amount of waste in a cost-effective way, thereby increasing its commercial value, said one of the lead researchers, Jin Chuan Wu, from the A*STAR Institute of Chemical and Engineering Sciences.
New engineered metabolic pathways in yeast enable efficient fermentation of xylose from biomass
March 05, 2015
Researchers with the Energy Biosciences Institute (EBI), a partnership that includes Berkeley Lab and the University of California (UC) Berkeley, have introduced new metabolic pathways from the fungus Neurospora crassa into the yeast Saccharomyces cerevisiae to increase the fermentative production of fuels and other chemicals from biomass. An open access paper on the work is publised in the journal eLife.
While S. cerevisiae is the industry mainstay for fermenting sugar from cornstarch and sugarcane into ethanol, it requires substantial engineering to ferment sugars derived from plant cell walls such as cellobiose and xylose. The new metabolic pathways enable the yeast to ferment sugars from both cellulose (glucose) and hemicellulose (xylose)—the two major families of sugar found in the plant cell wall—efficiently, without the need of environmentally harsh pre-treatments or expensive enzyme cocktails.
Researchers identify peptide to bind LMNO to improve power and performance of cathodes in Li-ion batteries
February 12, 2015
Researchers at the University of Maryland, Baltimore County (UMBC) have isolated a peptide, a type of biological molecule, which binds strongly to lithium manganese nickel oxide (LMNO), a material that can be used to make the cathode in high-performance Li-ion batteries. The peptide can latch onto nanosized particles of LMNO and connect them to conductive components of a battery electrode, improving the potential power and stability of the electrode.
The researchers presented their results at the 59th annual meeting of the Biophysical Society, held 7-11 Feb.in Baltimore, Maryland.
Engineered yeast produces ethanol from three important cellulosic biomass components simultaneously; higher yields, lower cost
February 11, 2015
A team led by researchers from the University of Illinois at Urbana−Champaign has, for the first time, integrated the fermentation pathways of both hexose and pentose sugars from biomass as well as an acetic acid reduction pathway into one strain of the yeast Saccharomyces cerevisiae using synthetic biology and metabolic engineering approaches.
The engineered strain co-utilized cellobiose, xylose, and acetic acid to produce ethanol with a substantially higher yield and productivity than the control strains. The results showed the unique synergistic effects of pathway coexpression, the team reported in a paper in the journal ACS Synthetic Biology.
Researchers use DNA to stabilize sulfur cathode for high-performance Li-sulfur batteries
February 10, 2015
|DNA has a high concentration of heteroatoms, including oxygen, nitrogen and phosphorus, that can anchor soluble polysulfides to improve the cycling performance of Li/S batteries. Li et al. Click to enlarge.|
A team from the China University of Geosciences has taken a novel approach to stabilizing Lithium-sulfur batteries by functionalizing the carbon-sulfur cathode with DNA.
Experimental results reported in a paper accepted for publication in the RSC Journal of Materials Chemistry A showed that adding a fine adding amount of DNA into a carbon/sulfur composite enables a significant improvement to cyclic performance by anchoring the soluble polysulfides that lead to performance degradation. The DNA-decorated electrode offered a discharge capacity of 771 mAh·g-1 at 0.1 C after 200 cycles (retention 70.7% of the initial)—a three-fold enhancement in capacity retention over 200 cycles.
Harvard hybrid “bionic leaf” converts solar energy to liquid fuel isopropanol
Scientists from a team spanning Harvard University’s Faculty of Arts and Sciences, Harvard Medical School and the Wyss Institute for Biologically Inspired Engineering at Harvard University have developed a scalable, integrated bioelectrochemical system that uses bacteria to convert solar energy into a liquid fuel. Their work integrates water-splitting catalysts comprising earth-abundant components with wild-type and engineered Ralstonia eutropha bacteria to generate biomass and isopropyl alcohol. An open access paper describing their work is published in Proceedings of the National Academy of Sciences (PNAS).
Pamela Silver, the Elliott T. and Onie H. Adams Professor of Biochemistry and Systems Biology at HMS and an author of the paper, calls the system a bionic leaf, a nod to the solar water-splitting artificial leaf invented by the paper’s senior author, Daniel Nocera, the Patterson Rockwood Professor of Energy at Harvard University. (Earlier post.)
Researchers discover bacteria could be rich source of terpenes
December 24, 2014
Researchers at Kitasato University in Japan, Brown University in the US, and colleagues in Japan have found that bacteria could be a rich source of terpenes—natural compounds common in plants and fungi that can be used to make drugs, food additives, perfumes, and other products, including advanced fuels (earlier post, earlier post).
Terpenes are responsible for the essential oils of plants and the resins of trees. Since the discovery of terpenes more than 150 years ago, scientists have isolated some 50,000 different terpene compounds derived from plants and fungi. Bacteria and other microorganisms are known to make terpenes too, but they’ve received much less study. The new research, published in an open access paper in the Proceedings of the National Academy of Sciences, shows that the genetic capacity of bacteria to make terpenes is widespread.
DOE JBEI team boosts methyl ketone production from E. coli 160-fold; advanced biofuel or blendstock
December 02, 2014
In 2012, researchers at the US Department of Energy’s Joint BioEnergy Institute (JBEI) engineered Escherichia coli (E. coli) bacteria to overproduce from glucose saturated and monounsaturated aliphatic methyl ketones in the C11 to C15 (diesel) range from glucose. In subsequent tests, these methyl ketones yielded high cetane numbers, making them promising candidates for the production of advanced biofuels or blendstocks. (Earlier post.)
Now, after further genetic modifications of the bacteria, they have managed to boost the E.coli’s methyl ketone production 160-fold. A paper describing this work is published in the journal Metabolic Engineering.
UCLA researchers develop synthetic biocatalytic pathway for more efficient conversion of methanol to longer-chain fuels
November 18, 2014
Researchers at the UCLA Henry Samueli School of Engineering and Applied Science led by Dr. James Liao have developed a more efficient way to turn methanol into useful chemicals, such as liquid fuels, and that would also reduce carbon dioxide emissions. The UCLA team constructed a synthetic biocatalytic pathway that efficiently converts methanol under room temperature and ambient atmospheric pressures to higher-chain alcohols or other higher carbon compounds without carbon loss or ATP expenditure.
Building off their previous work in creating a new synthetic metabolic pathway for breaking down glucose that could lead to a 50% increase in the production of biofuels (earlier post), the researchers modified the non-oxidative glycolysis pathway to utilize methanol instead of sugar. An open-access paper on the research was published in the 11 Nov. edition of the Proceedings of the National Academy of Sciences.
JBEI researchers boost isopentenol output from E. coli; potential benefit for bio-gasoline
October 27, 2014
Researchers at the US Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI) have identified microbial genes that can improve both the tolerance and the production of isopentanol in engineered strains of Escherichia coli. Isopentenol is a five-carbon (C5) alcohol that is a highly promising candidate for biogasoline, but, like other short-chained alcohols, is toxic to E.coli at commercial levels of fuel production.
Aindrila Mukhopadhyay, a chemist who directs the host engineering program for JBEI’s Fuels Synthesis Division, led a study in which transcriptomic data and a synthetic metabolic pathway were used to identify several genes that not only improve tolerance but also production of isopentenol in E.coli. MetR, the methionine biosynthesis regulator, improved the titer for isopentenol production by 55%, while MdlB, the ABC transporter, facilitated a 12% improvement in isopentenol production.
Solazyme and Amyris receive Presidential Green Chemistry Challenge awards
October 16, 2014
The US Environmental Protection Agency (EPA) has announced the 5 winners of the 2014 Presidential Green Chemistry Challenge Awards, including biotechnology companies Amyris and Solazyme, Inc. Solazyme received the award for Greener Synthetic Pathways for its tailored oils produced from microalgal fermentation. Amyris received the Small Business award for its renewable hydrocarbon farnesane for use as diesel and jet fuel.
Amyris has engineered yeast to make the hydrocarbon farnesene via fermentation instead of ethanol. Farnesene is a building block hydrocarbon that can be converted into a renewable, drop-in replacement for petroleum diesel without certain drawbacks of first-generation biofuels. A recent lifecycle analysis estimated an 82% reduction in GHG emissions for farnesane, compared with the EPA baseline fossil diesel—including indirect effects.
BNL team devises new method to boost oil accumulation in plant leaves; implications for biofuel production
October 08, 2014
Researchers at DOE’s Brookhaven National Laboratory (BNL) have developed a new method to increase significantly the amount of oil accumulated in plant leaves, which could then serve as a source for biofuel production. Rather than adding genes, as some other research teams have done in their efforts to boost oil accumulation, the BNL method is based on is based on disabling or inactivating genes through simple mutations.
A series of detailed genetic studies revealed previously unknown biochemical details about plant metabolic pathways, including new ways to increase the accumulation of oil in leaves. Using these methods, the scientists grew experimental Arabidopsis plants (widely used as model organisms in plant biology), the leaves of which accumulated 9 wt % oil. This represented an approximately 150-fold increase in oil content compared to wild type leaves. A paper on their work is published in the journal The Plant Cell.
Researchers enhance yeast thermotolerance and ethanol tolerance; potential for significant impact on industrial biofuel production
October 03, 2014
The yeast Saccharomyces cerevisiae plays a central role in global biofuel production; currently, about 100 billion liters of ethanol are produced annually worldwide by fermentation of mainly sugarcane saccharose and corn starch by the yeast. There are also efforts underway to use the yeast with cellulosic biomass.
Boosting the yield and lowering the cost of fermentative production of biofuel would not only result in a significant immediate financial impact to commercial ethanol operations, but also support cost reductions that would be helpful to advance other advanced biofuels using the same or a similar pathway. However, boosting production has been gated by two key conditions: the ability of the yeast to tolerate higher temperatures, and the ability of the yeast to survive high concentrations of ethanol. Now, two new separate studies report progress on each of those fronts; the findings could have a significant impact on industrial biofuel production. Both papers are published in the current issue of the journal Science.
ARPA-E to award $60M to 2 programs: enhancing biomass yield and dry-cooling for thermoelectric power
October 02, 2014
|ARPA-E’s vision of advanced phenotyping to enhance biomass yield. Click to enlarge.|
The US Department of Energy’s Advanced Research Projects Agency-Energy (ARPA-E) will award up to $60 million to two new programs ($30 million each). The Transportation Energy Resources from Renewable Agriculture (TERRA) program (DE-FOA-0001211) seeks to accelerate biomass yield gains (especially energy sorghum) through automated, predictive and systems-level approaches to biofuel crop breeding. The Advanced Research In Dry cooling (ARID) program (DE-FOA-0001197) aims to develop low-cost, highly efficient and scalable dry-cooling technologies for thermoelectric power plants.
TERRA. ARPA-E posited that there is an urgent need to accelerate energy crop development for the production of renewable transportation fuels from biomass. While recent advances in technology has enabled the extraction of massive volumes of genetic, physiological, and environmental data from certain crops, the data still cannot be processed into the knowledge needed to predict crop performance in the field. This knowledge is required to improve the breeding development pipeline for energy crops.
German researchers boost algal hydrogen production five-fold using metabolic engineering approach
September 25, 2014
Scientists from the Max Planck Institutes for Chemical Energy Conversion and Coal Research and from the research group Photobiotechnology at Ruhr-Universität Bochum (RUB) have discovered a way of increasing the efficiency of hydrogen production in microalgae by a factor of five by using a combined metabolic engineering approach. An open access paper on their work is published in the RSC journal Energy & Environmental Science.
The genetic modifications resulting in the enhanced light-driven hydrogen production opens new avenues for the design of H2-producing organisms, which might lead to the design of an economically competitive hydrogen producing organism, the researchers suggest.
Researchers successfully engineer E. coli to produce renewable propane; proof-of-concept
September 03, 2014
Researchers from the University of Turku in Finland, Imperial College London and University College London have devised a synthetic metabolic pathway for producing renewable propane from engineered E. coli bacteria. Propane, which has an existing global market for applications including engine fuels and heating, is currently produced as a by-product during natural gas processing and petroleum refining. A paper on their work is published in Nature Communications.
The new pathway is based on a thioesterase specific for butyryl-acyl carrier protein (ACP), which allows native fatty acid biosynthesis of the Escherichia coli host to be redirected towards a synthetic alkane pathway. Although the initial yields were low, the team was able to identify and to add essential biochemical components in order to boost the biosynthesis reaction, enabling a the E. coli strain to increase propane yield, although the amounts are still far too low for commercialization.