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Cellulosic ethanol

[Due to the increasing size of the archives, each topic page now contains only the prior 365 days of content. Access to older stories is now solely through the Monthly Archive pages or the site search function.]

Navigant Research forecasts 58% growth in global biofuels consumption by 2022; biodiesel and drop-in fuels gain market share

February 05, 2014

In a new report, “Biofuels for Transportation Markets”, Navigant Research forecasts that global demand for biofuels in the road transportation sector will grow from representing almost 6% of the liquid fuels market in 2013 to roughly 8% by 2022. Of that 8%, 8% will consist of advanced drop-in fuels, according to the research firm. Navigant forecasts that global biofuels consumption in the road transportation sector will grow from more than 32.4 billion gallons per year (BGPY) in 2013 to more than 51.1 BGPY in 2022—an increase of 58%.

Overall, Navigant forecasts that global retail sales of all liquid fuels for the road transportation sector will grow from more than $2.6 trillion in 2013 to more than $4.5 trillion in 2022 (73% growth).

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DEINOVE produces ethanol at 9% titer with its optimized Deinococcus bacteria

January 16, 2014

DEINOVE, a technology company that designs, develops and markets a new generation of industrial processes based on optimized Deinococci bacteria, has produced ethanol at a titer of 9% via its fermentation of biomass sugars in 20L pre-industrial fermentors. In September 2012, the company had reported that its optimized strain of Deinococcus generated ethanol from wheat-based biomass with a titer of 3%. (Earlier post.)

The 9% content v/v (volume/volume)—equal to 7.2% wt/v (weight/volume)—exceeds the 5% alcohol content wt/v considered to be the threshold for industrial exploitation of a process for 2nd generation biofuels, the company said. The obtained performance is gradually approaching the maximum theoretical yield, the company added. The use of Deinoccoccus offers several benefits:

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Sandia study finds meeting RFS2 requirements unlikely without stronger enforcement mechanism; the importance of drop-in biofuels

January 06, 2014

Even if well-known technology, infrastructure, economic and political challenges in meeting the biofuel requirements of the RFS2 mandate are overcome, it is “highly unlikely” that the light-duty vehicle parc will be capable of consuming the RFS2 (Renewable Fuel Standard) mandated volumes of biofuels, according to a new analysis by a team from Sandia National Laboratory.

The Sandia researchers showed that the key to meeting the RFS2 targets is the fuel price differential between E85 fuel and conventional gasoline (low ethanol blends), so that E85 owners refuel with E85 whenever possible. In other words, RFS2 will be satisfied if gasoline becomes significantly more expensive than E85 on a per energy basis. This is, however, the opposite of historic pricing trends, and suggests that policy intervention of a stronger enforcement mechanism will be required to meet RFS2 targets by creating market conditions necessary for greater biofuel consumption.

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NREL/UGA study finds microbial enzyme digests cellulose ~2x fast as current leading commercial cellulase; implications for biofuels cost

January 04, 2014

Researchers at the Energy Department’s National Renewable Energy Laboratory (NREL) and the University of Georgia have discovered that an enzyme from a microorganism first found in the Valley of Geysers on the Kamchatka Peninsula in Russia in 1990 can digest cellulose almost twice as fast as Cel7A, the current leading component cellulase enzyme on the market.

The high-performance enzyme CelA was discovered 15 years ago, but until this recent work, all that was known about this complex protein was its general architecture and that it had the ability to degrade cellulose. If it continues to perform well in larger tests, it could help drive down the price of making lignocellulosic fuels, from ethanol to other biofuels that can be dropped into existing infrastructure. A paper reporting this finding appears in the journal Science.

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Field trials with genetically modified poplars shows potential for efficient conversion to sugars but with impact on biomass yield

December 31, 2013

Vanacker
Ethanol yield (g/L) for the Belgian and French field trials. Van Acker et al. Click to enlarge.

The results of field trials with genetically modified poplar trees in Belgium and France shows that the wood of the modified poplar trees—down-regulated for cinnamoyl-CoA reductase (CCR), an enzyme in the lignin biosynthetic pathway—improved saccharification yield—i.e., it can be more efficiently converted into sugars for producing bio-based products such as bio-plastics and bio-ethanol.

However, the study, published as an open access paper in Proceedings of the National Academy of Sciences (PNAS), also found that strong down-regulation of CCR also affected biomass yield. The team, from Belgium, France and the US, led by researchers from VIB and Ghent University, concluded that CCR down-regulation may become a successful strategy to improve biomass processing if the yield penalty can be overcome.

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ICCT suggests minor changes to Fed tax policy to cut higher investment risk of 2nd-gen biofuels and advance the industry

December 22, 2013

Minor changes to an existing Federal tax incentive for second-generation biofuels (i.e., biofuel made from cellulose, algae, duckweed, or cyanobacteria) could mitigate the current elevated risk of investing in the industry that is retarding its advance, according to a new paper by a team from the International Council on Clean Transportation (ICCT) and Johns Hopkins University. Some of the ICCT recommendations are mirrored in the recently released Baucus draft proposal for tax reform (earlier post), notes Dr. Chris Malins of the ICCT, one of the study’s co-authors.

Previous studies have attempted to explain the slow commercialization of cellulosic and algal biofuels qualitatively, however few have presented financial analysis across the sector, the authors observe. Using publicly available financial data, they applied investment analysis tools (the capital assets pricing model, CAPM) that are generally not applied to this space in order to develop a more rigorous understanding of the investment risk in the industry.

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DSM and DONG Inbicon show cellulosic bio-ethanol fermentation on industrial scale with 40% higher yield

December 09, 2013

Royal DSM, together with DONG Energy (Denmark), has demonstrated the combined fermentation of C6 and C5 sugars from wheat straw on an industrial scale. The combined fermentation results in a 40% increase in ethanol yield per ton of straw, which can result in significant cost cuts in the production of bio-ethanol from cellulosic feedstock.

The demonstration took place in DONG Energy’s Inbicon demonstration plant in Kalundborg (Denmark), the longest running demonstration facility for cellulosic bio-ethanol production in the world. (Earlier post.) The facility was reconstructed in 2013 in order to be able to conduct mixed fermentation of C6 and C5 sugars. In a two-month fermentation test mixed C6 and C5 fermentation using DSM’s advanced yeast was found to yield 40% more ethanol per ton of straw than traditional C6 fermentation.

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Study shows bamboo ethanol in China technically and economically feasible, cost-competitive with gasoline

December 01, 2013

Bamboo, the composition of which is highly similar to energy grasses used for biofuel production such as switchgrass, is an interesting potential feedstock for advanced bioethanol production in China due to its natural abundance, rapid growth, perennial nature and low management requirements.

Now, researchers at Imperial College London have shown that bioethanol production from bamboo in China is both technically and economically feasible, as well as cost-competitive with gasoline. An open access paper on their study is published in Biotechnology for Biofuels.

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Raízen breaks ground on Iogen cellulosic ethanol facility in Brazil

November 29, 2013

Iogen Corporation announced that Brazilian ethanol giant Raízen Energia Participações S/A has started construction of a commercial biomass-to-ethanol facility using Iogen Energy’s advanced cellulosic biofuel technology. (Iogen Energy is a joint venture between Raízen and Iogen Corporation. Earlier post.)

The $100-million plant, to be located adjacent to Raízen’s Costa Pinto sugar cane mill in Piracicaba, São Paulo, will produce 40 million liters (10.6 million gallons US) of cellulosic ethanol a year from sugarcane bagasse and straw. Plant start-up is anticipated in the fourth quarter of 2014.

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EPA proposes reduction in cellulosic biofuel and total renewable fuel standards for 2014

November 15, 2013

The US Environmental Protection Agency (EPA) is proposing a reduction in the cellulosic biofuel and total renewable fuel standards (RFS) for 2014. Once the proposal is published in the Federal Register, it will be open to a 60-day public comment period.

Specifically, EPA is proposing a total renewable fuel target of 15.21 billion gallons; the final 2013 overall volumes and standards require 16.55 billion gallons; the original target as specified in the Clean Air Act is 18.15 billion gallons. (Earlier post.) EPA is setting the troublesome cellulosic biofuel target at 17 million gallons—significantly lower than the Clean Air Act (CAA) target of 1.75 billion gallons—but an increase from the 6.0 million gallons specified for 2013. This reflects EPA’s current estimate of the amount of cellulosic biofuel that will actually be produced in 2014, but EPA will consider public comments before setting the final cellulosic standard.

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Univ. of Illinois team argues that renewable fuel standard needs to be modified, not repealed

October 16, 2013

A policy analysis by two University of Illinois researchers argues that Congress should minimally modify, not repeal, the Renewable Fuel Standard (RFS). In the study, law professor Jay P. Kesan and Timothy A. Slating, a regulatory associate with the Energy Biosciences Institute, argue that RFS mandates ought to be adjusted to reflect current and predicted biofuel commercialization realities; that its biofuel categories be expanded to encompass all emerging biofuel technologies; and that its biomass sourcing constraints be relaxed.

In the paper, to be published in the NYU Environmental Law Journal, Kesan and Slating contend that the RFS can serve as a “model policy instrument” for the federal support of all types of socially beneficial renewable energy technologies.

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Yeast engineered to co-consume xylose and acetic acid boosts cellulosic ethanol yield by 10%

October 08, 2013

Commercial production of cellulosic biofuel via fermentation pathways has been hampered by inefficient fermentation of xylose and the toxicity of acetic acid, which constitute substantial portions of cellulosic biomass. Now, researchers from the University of Illinois at Urbana-Champaign and UC Berkeley have engineered yeast to convert cellulosic sugars and toxic levels of acetate together into ethanol under anaerobic conditions.

The innovation, reported in a paper published in Nature Communications, increases ethanol yield from lignocellulosic sources by about 10%. The results, the researchers suggest, demonstrate a breakthrough in making efficient use of carbon compounds in cellulosic biomass and also present an innovative strategy for metabolic engineering through which an undesirable redox state can be exploited to drive desirable reactions—even improving productivity and yield.

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Study finds HTL algal biofuels offer 50-70% lifecycle CO2 reduction compared to petroleum fuels; EROI and GHG comparable to or better than other biofuels

September 20, 2013

Liu
The EROI ratio and GHG emissions/MJ of (a) algae-derived diesel and (b) algae-derived gasoline produced using HTL. The results are benchmarked against commercialized biodiesel or bioethanol as well as petroleum-derived versions of the drop-in fuels. Credit: Liu et al. Click to enlarge.

A new life cycle analysis by a team led by researchers at the University of Virginia has concluded that biofuel produced from algae via hydrothermal liquefaction (HTL) can reduce life cycle CO2 emissions by 50 to 70% compared to petroleum fuels, and also has energy burdens and GHG (greenhouse gas) emission profiles that are comparable to or better than conventional biofuels, cellulosic ethanol and soybean biodiesel.

HTL algae-derived gasoline has a considerably lower GHG footprint and a better EROI relative to conventional ethanol made from corn on a per MJ basis, the team found. The data suggest that a shift to algae-derived gasoline could have immediate climate benefits even using existing technologies, the authors noted. In addition, given expected technological improvements, the benefits of algae-derived gasoline will likely improve.

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Novozymes and Raízen to collaborate on cellulosic ethanol in Brazil

September 18, 2013

Novozymes has entered into a collaboration agreement with Brazil’s largest sugarcane crusher, Raízen Energia S/A (the $12-billion joint venture between Shell and Cosan founded in 2011). (Earlier post.) As part of the agreement, Novozymes will supply enzyme technology to Raízen’s first commercial-scale cellulosic ethanol plant in Brazil, scheduled to be operational by end 2014.

The plant will be a bolt-on facility to Raízen’s Costa Pinto sugarcane mill in the state of São Paulo and will have the capacity to produce 40 million liters (10.5 million gallons US) of cellulosic ethanol a year from sugarcane bagasse and straw. The agreement also provides for Novozymes to supply enzyme technology to Raízen’s second cellulosic ethanol plant, should such a plant be constructed.

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JBEI team develops one-pot, wash-free process for pretreatment and saccharification of switchgrass; avenues for driving down biofuel cost

August 14, 2013

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Conventional separate pretreatment and saccharification of biofuel feedstock (a) entails water and waste disposal that the new one-pot system (b) eliminates. (Image courtesy of Joint BioEnergy Institute). Click to enlarge.

Researchers with the US Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI) report the first demonstration of a one-pot, wash-free process that combines ionic liquid (IL) pretreatment and enzymatic saccharification into a single vessel using a thermostable IL-tolerant bacterial consortium comprising several species of thermophiles (microbes that thrive at extremely high temperatures and alkaline conditions).

Using this one-pot system, they liberated 81.2% glucose and 87.4% xylose (monomers and oligomers) at 72 h processing at 70 °C with an enzyme loading of 5.75 mg g−1 of biomass at 10% [C2mim][OAc]. Glucose and xylose were selectively separated by liquid–liquid extraction with over 90% efficiency, thus eliminating extensive water washing as a unit operation.

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EPA sets 2013 percentages for Renewable Fuel Standard; anticipating adjustments to 2014 volume requirements

August 06, 2013

The US Environmental Protection Agency (EPA) finalized the 2013 percentage standards for four fuel categories that are part of the Renewable Fuel Standard (RFS) program. The final 2013 overall volumes and standards require 16.55 billion gallons of renewable fuels to be blended into the US fuel supply (a 9.74% blend).

The 2013 standard specifically requires: biomass-based diesel (1.28 billion gallons; 1.13%); advanced biofuels (2.75 billion gallons; 1.62%); and cellulosic biofuels (6.00 million gallons; 0.004%). These standards reflect EPA’s updated production projections. All volumes are ethanol-equivalent, except for biomass-based diesel which is actual volume.

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CCST report: an integral role for next-gen biofuels in meeting California GHG targets requires advanced biofuels and demand reduction

June 11, 2013

Next-generation biofuels can reduce greenhouse gas emissions of transportation to meet California’s target greenhouse gas (GHG) reduction goal, but deep replacement of fossil fuels through implementation of low-carbon lignocellulosic ethanol and advanced biomass derived hydrocarbons (drop-in biofuels) and reduction in demand is required, according to a new report from the California Council on Science and Technology (CCST).

The study, “California Energy Future: the Potential for Biofuels,” co-authored by Energy Biosciences Institute (EBI) scientists Heather Youngs and Chris Somerville, is the seventh and final report in its California’s Energy Future (CEF) project. The CEF project seeks ways the State could meet the mandated reductions of greenhouse gas (GHG) emissions to 80% below 1990 levels by 2050, exploring possible energy strategies for California through in-depth examinations of different technology scenarios.

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Anglo-Brazilian JV to launch first commercial bagasse cellulosic ethanol production plant in Brazil

April 14, 2013

UK-based TMO Renewables (TMO) and Usina Santa Maria Ltda have entered into an agreement to form a joint venture to build the first commercial production plant in Brazil to convert sugar cane waste (bagasse) to cellulosic bioethanol.

TMO signed a binding Memorandum of Understanding (MOU) with Usina Santa Maria Ltda to build Brazil’s first cellulosic bioethanol production facility in São Paulo state. Under the MOU, TMO in joint venture with Usina Santa Maria Ltda will first build, own and operate a 10-million liter (2.6-million gallon US) second-generation ethanol pilot plant to convert bagasse to cellulosic bioethanol.

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EBEI researchers shed light on how multiple cellulase enzymes attack cellulose; potential avenue to boosting sugar yields for biofuels

April 08, 2013

Blanch-Fig-1-Web1
PALM enables researchers to quantify how and where enzymes are binding to the surface of cellulose in heterogeneous surfaces, such as those in plant cell walls. Source: Berkeley Lab. Click to enlarge.

Researchers with the Energy Biosciences Institute, University of California, Berkeley have provided insight into how multiple cellulase enzymes attack cellulose, potentially yielding a way to improve the collective catalytic activity of enzyme cocktails that can boost the yields of sugars for making fuels.

Increasing the sugar yields from cellulosic biomass to help bring down biofuel production costs is essential for the widespread commercial adoption of these fuels. A paper on their work is published in Nature Chemical Biology.

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Navigant forecasts global 6% CAGR for biofuels to 2023

March 29, 2013

Navigant
Total Biofuels production by fuel type, world markets: 2013-2023. Source: Navigant. Click to enlarge.

Navigant Research forecasts global biofuels production will grow at a compound annual growth rate (CAGR) of 6% between 2013 and 2023, despite slower than expected development of advanced biofuels pathways (such as cellulosic biofuels); an expected expansion in unconventional oil production in key markets such as the United States; and a decline in global investment for biofuels in recent years.

In contrast, Navigant expects the CAGR for fossil-based gasoline, diesel, and jet fuel to be 3.1% over the forecast period. The research firm projects that total biofuels production will reach 62 billion gallons by 2023 or 5.9% of global transportation fuel production from fossil sources.

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JBEI team develops new one-pot process to extract biomass sugars from ionic liquid solutions

March 21, 2013

Ning
Process of biomass pretreatment, acid hydrolysis and sugar extraction using alkaline solutions. Sun et al. Click to enlarge.

A team from the Joint BioEnergy Institute (JBEI), Lawrence Berkeley National Laboratory has developed a novel one-pot process to extract sugars liberated from biomass in aqueous ionic liquid (IL) solutions. The new approach, described in an open access paper in the journal Biotechnology for Biofuels, potentially could significantly reduce costs of sugar production from lignocellulose by eliminating the need for costly enzymes and decreasing the water consumption requirements.

Many recent research and development efforts for cellulosic biofuels have explored a two-step bioconversion process involving: 1) liberation of fermentable sugars from lignocellulose; and 2) conversion of sugars into fuels and/or chemicals by fermentation. However, easily liberating the sugars and other monomers from cellulosic biomass for conversion is one of the major challenges to the cost-effectiveness of cellulosic pathways.

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ZeaChem begins production of cellulosic chemicals and ethanol, advances toward commercialization

March 12, 2013

Zeachemc2
Zeachem’s C2 platform uses an acetogenic organism to ferment sugars to acetic acid, which is converted to ethanol. Source: Zeachem. Click to enlarge.

ZeaChem Inc. has produced commercial-grade cellulosic chemicals and ethanol at its 250,000 gallons per year (GPY) demonstration biorefinery in Boardman, Ore. The demonstration facility is intended to showcase the scalability of ZeaChem’s biorefining process and serve as a stepping-stone toward large-scale commercial production.

Similar to a petrochemical refinery that makes multiple fuels and chemicals, ZeaChem’s demonstration facility is employing its C2 (two-carbon) platform to produce cellulose-based ethanol and intermediate chemicals such as acetic acid and ethyl acetate. (Earlier post.) The commercial market potential for all C2 products is $485 billion.

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Codexis introduces next-generation Codexyme cellulase enzymes with improved performance for reduced costs

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Codexis has delivered significant improvements in enzyme performance (left) and enzyme manufacturing cost (right). Source: Codexis. Click to enlarge.

Codexis, Inc., a developer of engineered enzymes for pharmaceutical, biofuel and chemical production, launched CodeXyme 4 and CodeXyme 4X cellulase enzyme packages for use in producing cellulosic sugar for production of biofuels and bio-based chemicals.

Codexis’ latest generation of advanced cellulase enzymes, CodeXyme 4 for dilute acid pretreatments and CodeXyme 4X for hydrothermal pretreatments, converts up to 85% of available fermentable sugars at high biomass and low enzyme loads. Combined with high strain productivity using the CodeXporter enzyme production system, this allows for a cost-in-use that the company believes will be among the lowest available once in full-scale commercial production.

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EIA: cellulosic biofuels will likely remain well below EISA targets

February 26, 2013

Biomasscap
Planned cellulosic biofuel production by 2015. Source: EIA. Click to enlarge.

US Commercial-scale production of cellulosic biofuels reached about 20,000 gallons in late 2012, according to the US Energy Information Administration (EIA). EIA estimates this output could grow to more than 5 million gallons this year, as operations ramp up at several plants. Additionally, several more plants with proposed aggregate nameplate capacity of around 250 million gallons could begin production by 2015, EIA said.

However, although cellulosic biofuels volumes are expected to grow significantly relative to current levels, they will likely remain well below the targets envisioned in the Energy Independence and Security Act of 2007 (EISA). EISA set a target level of 500 million gallons of cellulosic biofuels for 2012 and 1 billion gallons for 2013, growing to 16 billion gallons by 2022.

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