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[Due to the increasing size of the archives, each topic page now contains only the prior 365 days of content. Access to older stories is now solely through the Monthly Archive pages or the site search function.]

JBEI, UCSD scientists develop systems biology-based workflow to improve biofuels productivity

May 21, 2016

Researchers at the US Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI), in collaboration with researchers at the University of California, San Diego, have developed a workflow that integrates various “omics” data and genome-scale models to study the effects of biofuel production in a microbial host.

The development of omics technologies, such as metabolomics and proteomics, and systems biology have significantly enhanced the ability to understand biological phenomena. Nevertheless, the interpretation of large omics data into meaningful “knowledge” as well as the understanding of complex metabolic interactions in engineered microbes remains challenging. This new open-source workflow—which integrates various omics data and genome-scale models—drives the transition from vision to conception of a designed working phenotype.

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New renewable hydrocarbon fuel pathway uses platform molecule acetoin produced by biomass fermentation

March 30, 2016

Researchers at Nanjing Tech University in China have developed a new pathway for the production of liquid hydrocarbon fuels from lignocellulose. The new Nanjing Tech process uses acetoin—a novel C4 platform molecule derived from new ABE (acetoin–butanol–ethanol)-type fermentation via metabolic engineering—as a bio-based building block for the production of the liquid hydrocarbon fuels.

In a paper published in the RSC journal Green Chemistry, the Nanjing Tech team reported producing a series of diesel or jet fuel range C9–C14 straight, branched, or cyclic alkanes in excellent yields by means of C–C coupling followed by hydrodeoxygenation reactions.

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Berkeley Lab researchers devise new technique to reduce lignin and increase sugar yields; lowering biomass pretreatment costs

February 25, 2016

Scientists from the US Department of Energy’s Lawrence Berkeley National Laboratory (Berkeley Lab) and Joint BioEnergy Institute have devised a new strategy for reducing lignin in plants by modifying a key metabolic entrypoint for the synthesis of the most important lignin monomers.

The new technique, reported in an open-access paper in the journal Plant & Cell Physiology, could help lower the cost of converting biomass into lower carbon biofuels and bio-products.

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UCR team advances direct production of chemical and fuel precursors in yeast

January 28, 2016

A team led by a researcher at the University of California, Riverside has adapted the CRISPR-Cas9 gene editing system for use in a yeast strain that can produce useful lipids and polymers. The development will lead to new precursors for biofuels, specialty polymers, adhesives and fragrances.

Published recently in an open-access paper in the journal ACS Synthetic Biology, the research involves the oleaginous (oil-producing) yeast Yarrowia lipolytica, which is known for converting sugars to lipids and hydrocarbons that are difficult to make synthetically. Until now, Y. lipolytica has been hard to manipulate at the genetic level, but the application of CRISPR-Cas9 will change that, allowing scientists to tap into its bio-manufacturing potential.

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MSU researchers fabricate synthetic protein that streamlines carbon fixing machinery of cyanobacteria; potential boost for biofuels

September 22, 2015

Researchers at the MSU-DOE Plant Research Laboratory, Michigan State University, have fabricated a synthetic protein that not only improves the assembly of the carbon-fixing factory of cyanobacteria (also known as blue-green algae), but also provides a proof of concept for a device that could potentially improve plant photosynthesis or be used to install new metabolic pathways in bacteria.

The multi-function protein, which the researchers compare to a Swiss Army Knife, streamlines the molecular machinery of cyanobacteria, making biofuels and other green chemical production from these organisms more viable. The researchers describe their work in a paper in the journal The Plant Cell.

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Synbio company Intrexon and Dominion partner to commercialize bioconversion of natural gas to isobutanol in Marcellus and Utica Basins

August 20, 2015

Intrexon Energy Partners (IEP), a joint venture of synthetic biology company Intrexon Corporation and external investors (earlier post), and Dominion Energy, a subsidiary of Dominion Resources, have entered into an agreement to explore the potential for commercial-scale biological conversion of natural gas to isobutanol in the Marcellus and Utica Shale Basins.

Intrexon’s proprietary methanotroph bioconversion platform uses optimized microbial cell lines to convert natural gas into higher carbon compounds such as isobutanol and farnesene under ambient temperatures and pressures. This novel approach avoids costly, resource-intensive thermochemical gas-to-liquids (GTL) conversion methods, and offers a biofuel that does not utilize sugar or other plant-based feedstock.

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“Energiewende” in a tank; Audi e-fuels targeting carbon-neutral driving with synthetic fuels from renewables, H2O and CO2; Swiss policy test case

June 12, 2015

Like other major automakers, Audi (and its parent Volkswagen Group) is working on meeting its medium-term regulatory requirements (e.g., in the 2020 timeframe) by reducing the average fuel consumption of its new vehicles using a combination of three primary measures: optimizing its combustion engines for greater efficiency; developing alternative drive concepts, such as hybrid, plug-in hybrid and gas-powered vehicles; and reducing total vehicle weight through lightweight construction with an intelligent multimaterial mix.

Unlike the others, however, Audi over the past few years has embarked on a comprehensive approach to developing a range of new CO₂-neutral fuels as part of its overall strategy for sustainable, carbon-neutral mobility: Audi e-fuels. Audi’s basic goal is to combine renewable energy (e.g. solar and wind), water and CO2 to produce liquid or gaseous fuels with a very low carbon intensity. Audi e-fuels are intended to use no fossil or biomass sources; do not compete with food production; and are 100% compatible with existing infrastructure.

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Delivery of renewable isooctane to Audi tips interesting potential non-biomass pathway for biogasoline; “e-benzin” as solar fuel

May 26, 2015

Last week, Audi and its partner Global Bioenergies announced that the first batch of renewable isooctane—which Audi calls “e-benzin”—using Global Bioenergies’ fermentative isobutene pathway (sugar→isobutene→isooctane) had been produced and presented to Audi by Global Bioenergies. (Earlier post.)

Global Bioenergies, founded in 2008, has developed a synthetic isobutene pathway that, when implanted in a micro-organism, enables the organism to convert sugars (e.g., from starch and biomass) via fermentation into gaseous isobutene via a several-stage enzymatic process. However, following the delivery of the first renewable isooctane, Reiner Mangold, Audi’s head of sustainable product development, said that Audi was “now looking forward to working together with Global Bioenergies on a technology allowing the production of renewable isooctane not derived from biomass sources”—i.e., using just water, H2, CO2 and sunlight.

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