|Chrysosporium lucknowense is the basis for Dyadic’s enzyme work.|
Dyadic International, a biotechnology company, will report today at the Third Annual World Congress on Industrial Biotechnology and Bioprocessing in Toronto, Canada, that it has identified and tested highly effective enzyme mixtures for the efficient conversion of renewable cellulosic biomass to ethanol.
Dyadic scientist Marco Baez, Ph.D. is presenting results of an internal study on Dyadic’s proprietary cellulase and hemicellulase mixes with strong saccharifying activity on a number of different lignocellulosic feedstocks, including Douglas fir and cotton.
In addition, Dr. Baez will present data showing the results of an internal study of two new highly active cellobiohydrolases isolated from Dyadic’s patented Chrysosporium lucknowense fungal strain (known as C1), as well as a mixture of pure monocomponent enzymes, all of which demonstrated an extremely high ability to convert different cellulosic substrates to glucose to then be fermented into ethanol.
We are making meaningful strides toward the production of low-cost ethanol from biomass. The key hurdles that must be overcome for the large-scale commercial production of low-cost ethanol from renewable biomass are to reduce the cost and increase the efficiency of the enzymes used to extract glucose and other sugars from the cellulolytic feedstocks.
The results to be presented today show that we are making rapid progress in the development of potent enzyme mixtures that work well in the cellulosic ethanol application. Dyadic has filed a patent application with the US Patent Office for these new enzymes and mixtures containing these enzymes.—Dr. Glenn Nedwin, Chief Science Officer
Separately, Dyadic noted that in a recent publication (BC Saha and M.A. Cotta, Biotech. Prog. 22:449-453, 2006), researchers at the US Department of Agriculture measured the efficiency of various commercially available enzyme preparations (including Dyadic’s Viscostar 150L) on wheat straw pretreated with alkaline peroxide for the extraction of glucose and other sugars.
...unlike corn fiber hemicellulose, which is very resistant to hydrolysis using commercial enzymes, wheat straw hemicellulose can be easily hydrolyzed enzymatically by using a single xylanase preparation (Viscostar) after alkaline peroxide treatment.—Badal Saha and Michael Cotta
Dyadic is also investigating applying its C1 and other technologies to develop enzymes to convert distillers dried grains—DDG, a byproduct of corn ethanol production—into fermentable sugars to further extend the ethanol yield per bushel of corn. Dyadic estimates it can deliver a 10-20% yield improvement.
Ethanol Production from Alkaline Peroxide Pretreated Enzymatically Saccharified Wheat Straw; Badal C. Saha and Michael A. Cotta