Projects were selected for the organism’s capacity to convert lignocellulosic biomass to ethanol—i.e., to ferment C5 and C6 sugars—in process-relevant conditions that would be economical in the commercial market.  Additionally, the organism must be able to survive a wide range of environmental conditions and remain stable from adverse mutation.

Base strains that could be adapted for specific process schemes are needed on a widely available basis. Such strains need to be able to convert a wide range of sugars at rates, yields and titers commensurate with production strain requirements and tolerate the potentially inhibitory environment of pretreated lignocellulosic biomass.

From an analysis of the present status of technology and future targets regarding the use of biomass hydrolyzates for ethanol production, the following are critical parameters necessary for the development of a cost-competitive process:

1. High yield or, equivalently, full sugar utilization with minimal byproduct formation;

2. High final ethanol titer;

3. High overall volumetric productivity;

4. Tolerance to inhibitors present in hydrolyzates; and

5. Affordable microbial systems.

—from the Funding Opportunity Document

Combined with the industry cost share, more than $37 million could be invested in these five projects.  Negotiations between the selected companies and DOE will begin immediately to determine final project plans and funding levels.  Funding will begin this fiscal year and run through FY 2010, subject to congressional appropriations.

Projects submitted by these five applicants were selected:

1. Cargill Incorporated to receive up to $4.4 million;

2. Celunol Corporation to receive up to $5.3 million;

3. E.I. Dupont de Nemours & Company to receive up to $3.7 million;

4. Mascoma Corporation to receive up to $4.9 million; and

5. Purdue University to receive up to $5.0 million.

Resources:

• Development of Robust, Highly Efficient Fermentative Organisms for the Conversion of Lignocellulosic Biomass to Ethanol; Funding Opportunity Number: DE-PS36-07GO97002