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Sandia-led team demonstrates two efficient hybrid biochemical routes for lignin conversion to value-added chemicals

A Sandia National Laboratories-led team has demonstrated two new routes to lignin conversion that combine the advantages of earlier methods while minimizing their drawbacks. The team’s recent findings are described in an open access paper in the journal Scientific Reports.

Lignin, the tough material left over from biofuel production, contains compounds that can be converted into products such nylon, plastics and drugs. Lignin is one of the main components of plant cell walls, and gives plants structural integrity as well as protection from microbial attacks. Products made from converted lignin could subsidize biofuel production, making the cost of biofuels more competitive with petroleum. Unfortunately, lignin’s toughness also makes it difficult to extract its valuable compounds. Scientists have wrestled for decades with deconstructing it. As a result, lignin often sits unused in giant piles.

To break the bonds between compounds that make up lignin, scientists have either employed chemicals or microorganisms such as bacteria or fungi. The gentler biological methods do enable the production of specific targeted compounds; however, fully breaking down lignin using this approach can take weeks or even months.

Conversely, harsh chemicals can deconstruct lignin in hours or even minutes. But this method requires expensive catalysts and is sometimes toxic, and therefore unsustainable. Worse, chemical methods lead to a mixture of compounds that each appear in extremely small quantities.

You get a little bit of whole lot of various chemicals when you break down lignin this way. The quantities yielded are not terribly useful.

—Sandia bioengineer Seema Singh

Singh’s team has demonstrated two new techniques that incorporate the speed of a chemical method and the precision of a biological one. In both cases, Singh’s team ultimately produced high-value chemicals that currently are derived only from petroleum: muconic acid and pyrogallol.

Muconic acid can easily be turned into nylon, plastics, resins or lubricants, and pyrogallol has anti-cancer applications. Together, Singh reports, these chemicals have a combined market value of $255.7 billion.

The team’s first new conversion method is a multi-stage process that begins by pre-treating lignin with a weak solution of hydrogen peroxide and water. Intermediary molecules vanillin and syringate result from the treatment.

A strain of E. coli specially modified by Sandia microbiologist Weihua Wu then consumes these middle-stage compounds, several additional compounds emerge in the mix, and ultimately the process results in the two final chemicals.

However, Singh was not satisfied with the amount of muconic acid yielded from this process. So, she and her team challenged themselves to find a way to maximize their muconic acid yield, and tested a second conversion method.

The second method skips the process of having to break down the lignin altogether. Instead, the team genetically engineered a tobacco plant. As it grows, the plant produces high amounts of intermediate compound protocatechuate, known as PCA. Then, the only steps remaining were to extract that compound and use the engineered E. coli to make the muconic acid.

We basically skipped three-quarters of the steps we were doing previously by engineering the plant to grow intermediate chemicals. PCA can be easily extracted from the modified tobacco and converted into muconic acid with little effort.

—Seema Singh

This plant engineering route is not only more efficient, but it also successfully solves the team’s self-imposed challenge of maximizing muconic acid yield by as much as 34% over previous conversion methods.

Sandia funded the majority of the work on this project through its Laboratory Directed Research and Development program. The tobacco plant engineering work was done by Singh’s collaborators from the feedstock division at the Joint BioEnergy Institute in Emeryville, Calif., including Dominique Loque and Aymerick Eudes.

Singh directs the biomass pretreatment program at the institute, which is staffed by scientists from a consortium of laboratories including Lawrence Berkeley National Laboratory. She believes future research into increasing lignin’s economic value will be heavily influenced by her team’s demonstrations.

The biggest challenge in this field will be further maximizing the yield of valuable chemicals and the rate at which they can be yielded. Industrial adoption of this technology will depend on the ability to quickly produce large amounts of high-value product.

Everyone understands that hybrid approaches are key to lignin valorization. If you can only make milligram amounts in a month from a bug, that just won’t cut it. You want the organisms to make kilogram amounts in less than an hour, ideally.

—Seema Singh


  • Weihua Wu, Tanmoy Dutta, Arul M. Varman, Aymerick Eudes, Bianca Manalansan, Dominique Loqué & Seema Singh (2017) “Lignin Valorization: Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals” Scientific Reports 7, Article number: 8420 doi: 10.1038/s41598-017-07895-1


fred schumacher

During the last eruption of Minnesota forest tent caterpillars (Malacosoma disstria), I noticed that the egg cases were composed of some kind of hard, light aerogel. Since the adults only live for a day or two, just long enough to reproduce, I thought that whatever the substance was, it must have been produced in the body of the caterpillar and stored and since their primary food is aspen (Populus tremuloides) leaves, the most likely source of the egg case polymer would have been a resin, namely lignin, and that modification of that resin may have been done by bacteria in the gut of the caterpillar. It's a marvelous substance, strong and light with tremendous glue strength. It would be worth investigating tent caterpillars to see what is going on.

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